Biochemical Effects of High Concentration of Colocasia Esculenta Flour Fed on Streptozotocin-Induced Diabetic Rats

Uro-Chukwu, H.C *

Department of Biochemistry, Coal City University, Enugu, Nigeria ,Institute for Nutrition, Nutraceuticals & Public Health Research & Development, Nigeria and Department of Community Medicine, Ebonyi State University, Abakaliki, Nigeria.

Ezekwe, A.S

Department of Medical Biochemistry, Rivers State University, Nkporlu, Port Harcourt, Nigeria.

Roberts, F

Department of Medical Biochemistry, Rivers State University, Nkporlu, Port Harcourt, Nigeria.

Okari, K. A

Department of Medical Biochemistry, Rivers State University, Nkporlu, Port Harcourt, Nigeria.

Uro-Chukwu, F.N.C

Institute for Nutrition, Nutraceuticals & Public Health Research & Development, Nigeria.

*Author to whom correspondence should be addressed.


Background: An endocrine and metabolic disease of the pancreas such as Diabetes Mellitus (DM), is of Public health dimension responsible for 536.6 million cases in the globe. The treatment of DM takes lots of resources, making alternative treatment options like the utilization of medicinal plants like Colocasia Esculenta (CYN) a point of research, hence this study involving biochemical evaluation of streptozotocin-induced diabetic rats (SIDR) fed on high concentration of the flour.

Methodology: CYN was processed into pellets, dried in an oven at 60°C, and adequately stored for further use. Fourty-two male albino rats with weights ranging from 134 and 247 grammes, were purchased, acclimatized and induced with insulin resistance by the administration of  10% fructose diet, thereafter, the rats were made to develop type-2 diabetes mellitus (T2DM) by the intraperitoneal injection of  streptozotocin. For 28 days, an intervention formulation consisting of 50:50% ratio of cocoyam flour and commercial rat feed, was administered at the end of which blood samples were collected from the slaughtered animals for various biochemical analyses.

Results & Discussion: The active biological substances in the cocoyam flour included phenolics, D-stilbene, phthalate, and artemisinin, along with more antioxidant minerals than those found in usual rat meals. The formulation exhibited CAT activity of 7.9 units/min and DPPH of 53.2%. Poor glycemic control was suggested by the persistently elevated random blood glucose readings observed along the time trend. Although the results of the liver function tests were similar for the intervention and standard control, the formulation was more effective than metformin at reducing lipid peroxidation and in the hypolipidemic effects.

Conclusion: Administration of high cocoyam flour concentration demonstrated comparable biochemical effects to the anti-diabetic drug metformin in SIDRs, despite its poor glycemic control. This suggests that cocoyam flour may be applied as a supplemental treatment for people with T2DM.


Keywords: Cocoyam flour, glycemic control, diabetic rats

How to Cite

H.C , Uro-Chukwu, Ezekwe, A.S, Roberts, F, Okari, K. A, and Uro-Chukwu, F.N.C. 2024. “Biochemical Effects of High Concentration of Colocasia Esculenta Flour Fed on Streptozotocin-Induced Diabetic Rats”. International Research Journal of Gastroenterology and Hepatology 7 (1):51-67.


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Sun H, Saeedi P, Karuranga S, Pinkepank M, Ogurtsova K, Duncan BB, Stein C, Basit A, Chan JCN, Mbanya JC, Pavkov ME, Ramachandaran A, Wild SH, James S, Herman WH, Zhang P, Bommer C, Kuo S, Boyko EJ, Maqliano DJ. IDF Diabetes Atlas: Global, Regional and Country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Research and Clinical Practice. 2022; 183:109119. Available:

Van Belle TL, Coppieters KT, Von Herrath MG. Type 1 diabetes: Aetiology, immunology and therapeutic strategies. Physiological Reviews. 2011;91:1.

Lin X, Xu Y, Pan X, et al. Global, regional, and national burden and trend of diabetes in 195 countries and territories: An analysis from 1990 to 2025. Sci Rep. 2020;10:14790.

Kirigia JM, Sambo HB, Sambo LG, Barry SP. Economic burden of diabetes mellitus in the WHO African region. BMC International Health and Human Rights. 2009;9:6.

Ashcroft FM, Ashcroft SJH. Insulin, molecular biology to pathology. Oxford University Press. 1992;266-284.

Sobngwi E, Mauvais-Jarvis F, Vexiau P, Mbanya JC, Gautier JF. Diabetes in Africans. Epidemiology and clinical specificities. Diabetes Metab. 2001;27(6):628-634.

Piero MN. Hypoglycemic effects of some Kenyan plants traditionally used in the management of diabetes mellitus in the eastern province, MSc thesis, Kenyatta University; 2006.

Wylie-Rosett J, Delahanty LM. Nutrition in the Prevention and Treatment of Disease (Fourth Edition). 2017;691-707. Available:

Forouhi GN, Misra A, Mohan V, Taylor R, Yancy W. Dietary and nutritional approaches for prevention and management of type 2 Diabetes, Science and Politics of Nutrition, BMJ 361; 2018. Available:

Piero MN, Njagi JM, Kibiti CM, Ngeranwa JJN, Njagi ENM, Miriti PM. The Role of Vitamins and Mineral Elements in Management of Type 2 Diabetes Mellitus: A Review South As. J. Biol.Sci. 2012;2(Supp. 1):107 –115.

Merida LA, Mattos EB, Correa AC, Pereira PR, Paschoalin VM, Pinho MF, Vericimo MA. Tarin stimulates granulocyte growth in bone marrow cell cultures and minimizes immunosuppression by cyclo-phosphamide in mice. Plos One. 2018;13:e0206240.DOI: 10.1371/journal.pone.0206240

Chukwuma CI, Islam MS, Amonsou EO. A comparative study on the physicochemical, anti-oxidative, anti-hyperglycemic and anti-lipidemic properties of amadumbe (Colocasia esculenta) and okra (Abelmoschus esculentus) mucilage. J. Food Biochem. 2018;42:e12601.DOI: 10.1111/jfbc.12601

Prabhakar PK, Doble M. A target based therapeutic approach towards diabetes mellitus using medicinal plants. Current Diabetes Reviews. Bentham Science Publishers Ltd. 2008;291-308.

Kumawat NS, Chaudhari SP, Wani NS, Deshmukh TA, Patil VR. Antidiabetic activity of ethanol extract of Colocasia esculenta leaves in alloxan-induced diabetic rats. International Journal of Pharmaceutical Technique Research. 2010;2:1246–1249.

Eleazu CO, Iroaganachi M, Eleazu KC. Ameliorative potentials of cocoyam (Colocasia esculenta L.) and unripe plantain (Musa paradisiaca L.) on the relative tissue weights of streptozotocin-induced diabetic rats. J Diabetes Res. 2013;1–8


Eleazu CO, Eleazu KC, Iroaganachi MA. Effect of cocoyam (Colocasia esculenta), unripe plantain (Musa paradisiaca) or their combination on glycated haemoglobin, lipogenic enzymes, and lipid metabolism of streptozotocin-induced diabetic rats, Pharmaceutical Biology. 2016;54(1):91-97.DOI: 10.3109/13880209.2015.1016181

Shakya AK. Medicinal plants: Future source of new drugs International Journal of Herbal Medicine. 2016;4(4):59-64

NRC (National Research Council). Guide for the care and use of laboratory Animals. Bethesda (MD): National Institute of Health. 1985;8523.

Nair AB, Jacob S. A simple practice guide for dose conversion between animals and humans. J Basic Clinical Pharmacology. 2016;7(2):27-31. DOI: 10.4103/0976-0105.177703

Pneu-Dart. Dosage Calculation; 2023.

Available: Accessed 14th April 2024

Nnadi NN, Ezekwesili CN, Ezeigwe OC. Effects of formulated unripe plantain and millet dietary feeds in alloxan-induced diabetic albino rats. International Journal of Innovative Research and Advanced Studies (IJIRAS). 2022;9(6).

Tietz NW. Clinical Guide to Laboratory Tests. 3rd Edn. Philidelphia, PA: WB Saunders Company; 1995.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without the use of preparative ultracentrifuge. Clin Chem. 1972;18:499-505

Jin XH, Ohgami K, Shiratori K, Suzuki Y, Koyama Y, Yoshida K, Ilieva I, Tanaka T, Onoe K, Ohno S. Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo. Experimental Eye Research. 2006;82:860–867.DOI: 10.1016/j.exer.2005.10.024

Oulai AC, Dje KM, Eba KP, Adima AA, Kouadio EJP. Chemical composition, antioxidant and antimicrobial activities of capsicum annuum var. annuum concentrated extract obtained by reverse osmosis. GSC Biological and Pharmaceutical Sciences. 2018;05(02):116-125

Lalhminghlui K, Jagetia GC. Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth In vitro. Future Science. 2018;4(2):283.

Mukhtar A, Latif S, Rojas AAS, Muller J. Catalase activity in hot-air dried mango as an indicator of heat exposure for rapid detection of heat stress. Applied Sciences. 2022;12(3):1305. DOI: 10.3390/app12031305

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine. 1967;70:158–169.

Beutler E, Duron O, Kelly BM. Improwed method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine. 1963;61:882–888.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochemistry. 1979;95(2):351-358.

Wada, E., Feyissa, T., Tesfaye, K (2019) Proximate, Mineral and Antinutrient Contents of Cocoyam (Xanthosoma sagittifolium (L.) Schott) from Ethiopia. International Journal of Food Science, Vol. 2019. Article ID 8965476

Coronell-Tovar DC, Chavez-Jauregui RN, Bosques-Vega A, Lopez-Moreno ML. Characterization of cocoyam (Xanthosoma spp.) corn flour from the Nazareno cultivar. Food Science and Technology, Campinas. 2019;39(2):349-357.

Aini F, Maritsa H, Riany H. Antioxidant activity of nipahendophytic fungi (Nypha fruiticans Wurmb) from Tanjung Jabung Timur Jambi.Jurnal Biota. 2019;5:104- 109.

Kurniawan YS, Priyangga KTA, Krisbiantoro PA, Imawan AC. Green chemistry influences in organic synthesis: A review. Journal of Multidisciplinary Applied Natural Science. 2021;1:1-12.

Amrulloh H, Fatiqin A, Simanjuntak W, Afriyani H, Annissa A. Bioactivities of nanoscale magnesium oxide prepared using aqueous extract of Moringaoleifera leaves as a green agent. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2021;12:015006

Jiang Y, Shui J, Zhang B, Chin J, Yue R. The potential roles of artemisinin and its derivatives in the treatment of type 2 diabetes mellitus. Frontiers in Pharmacology. 2020;11:585487

Farrag EAE, Hammad MO, Safwat SM, Hamed S, Hellal D. Artemisinin attenuates type 2 diabetic cardiomyopathy of AGE-RAGE/HMGB-1 Signaling pathway. Scientific Reports, 13. Article 11043; 2023.

Maschirow L, Khalaf K, Al-Aubaidy HA, Jelinek HF. Inflammation, coagulation, endothelial dysfunction and oxidative stress in prediabetes – Biomarkers as a possible tool for early disease detection for rural screening. Clinical Biochemistry; 2015 Available:

Morel Y, Barouki. Repression of gene expression by oxidative stress. Biochemical Journal. 1999;342:481–496.

He X, Gao F, Hou J, Li T, Tan J, Wang C, Liu X, Wang M, Liu H, Chen Y. Metformin inhibits MAPK signalling and rescues pancreatic aquaporin 7 expression to induce insulin secretion in type 2 diabetes mellitus. Journal of Biological Chemistry. 2021;297.

Byrne NJ, Rajasekaran NS, Abel ED, Bugger H. Therapeutic potential of targeting oxidative stress in diabetic cardiomyopathy. Free Radical Biology and Medicine. 2021;169:317-342.

Rabhi H, Guermouche B, Merzouk H, Merzouk SA. The Mediterranean diet biodiversity impact on metabolic and oxidative stress parameters in type 2 diabetes. Genetics and Biodiversity. 2022;6(2):87-102.

El-Naggar SA, Elwan M, Abo M, Basyouny E, Elshennawy EO, El-Said K0S. Metformin Causes Hepato-renal Dysfunctions in Obese Male Rats. Brazilian Archives of Biology and Technology. 2021;64:e21210188.

Mak TW, Saunders ME. The immune response. Basic and clinical principles. In: Press EA, editor, San Diego, CA Elsevier Academic Press. 2006;464–516.

Dinarello CA. Proinflammatory cytokines. Chest. 2000;118:503–508.

Wong HL, Pfeiffer RM, Fears TR, Vermeulen R, Ji S, Rabkin CS. Reproducibility and correlations of multiplex cytokine levels in asymptomatic persons. Cancer Epidemiology, Biomarkers and Prevention. 2008;17: 3450–3456.

Shoelson SE, Lee J, Goldfine AB. Inflammation and insulin resistance. J. Clin. Invest. 2006;116(7):1793–1801. DOI: 10.1172/jci2906

Nakano S, Kuboki K, Matsumoto T, Nishimura C, Yoshino GSmall, dense LDL and high-sensitivity C-reactive protein (hs-CRP) in metabolic syndrome with type 2 diabetes mellitus. Journal of Atherosclerosis and Thrombosis. 2010;17(4):410–415

Fu W, Ma Y, Li L, Liu J, Fu L, Guo Y, Zhang Z, Li J, Jiang H. Artemether regulates metaflammation to improve glycolipid metabolism in db/db mice. Diabetes Metab. Syndr. Obes. 2020;13:1703–1713. DOI: 10.2147/dmso.S240786

Pickup JC, Chusney GD, Thomas SM, Burt D. Plasma interleukin-6, tumour necrosis factor alpha and blood cytokine production in type 2 diabetes. Life Sci. 2000;67(3):291–300. DOI: 10.1016/S0024-3205(00) 00622-6

Olcum M, Toston B, Ercan I, Eitutan IB, Genc S. Inhibitory effects of phytochemicals on NLRP3 inflammasome activation: A review. Phytomedicine. 2020;75:153238.Available:

Karlsen A, Retterstol L, Laake P, Paur I, Kjolsrud-Bohn S, Sandvik L, Blomhoff R. Anthocyanins inhibit nuclear factor-kappaB activation in monocytes and reduce plasma concentrations of pro-inflammatory mediators in healthy adults. Journal of Nutrition. 2007;137:1951–1954.

Hanley AJ, Williams K, Festa A. Elevations in markers of liver injury and risk of type 2 diabetes: The insulin resistance atherosclerosis study. Diabetes. 2004; 53(10):2623‐2632.

Giannini EG, Testa R, Savarino V. Liver enzyme alteration: A guide for clinicians. CMAJ. 2005;172(3):367‐379.

Lee SH, Parka HJ, Chuna HK, Choa SY, Junga HJ, Choa SM, Kimb DY, Kangb MS, Lillehojc HS. Dietary phytic acid improves serum and hepatic lipid levels in aged ICR mice fed a high-cholesterol diet. Nutrition Research. 2007;27(8):505-510.

Hanigan MH, Frierson HF. Immunohistochemical detection of gamma‐glutamyl transpeptidase in normal human tissue. The Journal of Histochemistry and Cytochemistry. 1996; 44(10):1101‐1108

Berk P, Korenblat K. Approach to the Patient with Jaundice or abnormal liver tests.In Goldman`s Cecil Medicine: 24th Edn, Vol.1: 956-966. Elsevier Inc; 2011.DOI: 10.1016/B978-1-4377-1604-7.00149-4

Wang Y, Koh WP, Yuan J, Pan A. Association between liver enzymes and incident type 2 diabetes in Singapore Chinese men and women. BMJ Open Diabetes Res Care. 2016;4(1):e000296.

Finelli C, Tarantino G. What is the role of adiponectin in obesity-related non‐alcoholic fatty liver disease? World J Gastroenterol WJG. 2013;19(6):802.

Yazdi HB, Hojati V, Shiravi A, Hosseinian S, Vaezi G, Hadjzadeh MA. Liver dysfunction and oxidative stress in streptozotocin-induced diabetic rats: Protective role of Artemisia Turanica. Journal of Pharmacopuncture. 2019;22(2): 109-114. DOI: 10.3831/KPI.2019.22.014

Islam S, Rahman S, Haque T, Sumon AH, Ahmed AM, Ali N. Prevalence of elevated liver enzymes and its association with type 2 diabetes: A cross-sectional study in Bangladeshi adults. American Journal of Physiology-Endocrinology and Metabolism. 2020;3(2).

Wu TW, Fung KP, Wu J, Yang CC, Weisel RD. Antioxidation of human low-density lipoprotein by unconjugated and conjugated bilirubin. Biochemical Pharmacology. 1996;51:859–862.

Troughton JA, Woodside JV, Young IS, Dominique A, Philippe A, Ferrie` res, et al. Bilirubin and coronary heart disease risk in the Prospective Epidemiological Study of Myocardial Infarction (PRIME). European Journal of Cardiovascular Prevention and Rehabilitation. 2007;14:79–84,DOI: 10.1097/01.hjr.0000230097.81202.9f (2007).

Gazzin S, Vitek L, Watchko J, Shapiro SM, Tiribelli C. A novel perspective on the biology of bilirubin in health and disease. Trends in Molecular Medicine. 2016;22(9):758–768.

Dong H, Huang H, Yun X, Kim D, Yue Y, Wu H, Sutter A, Chavin KD, Otterbein LE, Adams DB, Kim Y, Wang H. Bilirubin increases insulin sensitivity in leptin-receptor deficient and diet-induced obese mice through suppression of ER stress and chronic inflammation. Endocrinolog. 2014;155:818–828.DOI: 10.1210/en.2013-1667

Wang J, Li Y, Han X, Hu H, Wang F, et al. Serum bilirubin levels and risk of type 2 diabetes: Results from two independent cohorts in middle-aged and elderly Chinese, Scientific Reports. 2017;7:41338.

Wang G, Li W, Lu X, Zhao X, Xu L. Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats. Croatian Medical Journal. 2013;54:171-9.DOI: 10.3325/cmj.2013.54.171

Shlomo M, Polonsky KS, Larsen PR, Kronenberg HM. Diabetes Mellitus. Willams textbook of endocrinology, 12th Ed. Philadelphia: Elsevier/Saunders. 2011;1371-1435

Ali AA, Faris HA. Prevalence and determinants of microalbuminuria among type 2 diabetes mellitus patients, Baghdad, Iraq, 2013. SJKDT. 2016;27(2):348-355.

Yassine C, Maram M, Rahma M, Rania M, Mohamed J, Ines E, Hamadi F, Najet S, Naziha M, Jed J. Investigation of the renal protective effect of combined dietary polyphenols in streptozotocin-induced diabetic aged rats. Nutrients. 2022;14(14):2867.Available:

Andonova M, Petko D, Krastina T, Penka Y, Nikola K, Krasimira N, Veselin I, Krasimira G, Nikola N, Ilia T, Chernev C. Metabolic Markers Associated with Progression of Type 2 Diabetes Induced by High-Fat Diet and Single Low Dose Streptozotocin in Rats Veterinary Sciences. 2023;10(7):431.Available:https,//

Rana S, Dixit S, Mittal A. In silico target identification and validation for antioxidant and anti-inflammatory activity of selective phytochemicals. Braz. Arch. Boil. Technol. 2019;62(4).

Rudrapal M, Khairnar SJ, Khan J, Dukhyil AB, Ansari MA, Alomary MN, Alshabrmi FM, Palai S, Deb PK, Devi R. Dietary Polyphenols and Their Role in Oxidative Stress-Induced Human Diseases: Insights Into Protective Effects, Antioxidant Potentials and Mechanism(s) of Action. Front. Pharmacol. 2022;13: 806470.

Forbes JM, Fukami K, Cooper ME. Diabetic nephropathy: Where hemodynamics meets metabolism. Exp Clin Endocrinol Diabetes. 2007;115(2):69–84.

Cabral-Pacheco GA, Garza-Veloz I, Castruita-De la Rosa C, Ramirez-Acuña JM, Perez-Romero BA, Guerrero-Rodriguez JF, Martinez-Avila N, Martinez-Fierro ML. The Roles of Matrix Metalloproteinases and Their Inhibitors in Human Diseases. International. Journal of. Molecular. Science. 2020;21:9739.

Catania JM, Chen G, Parrish AR. Role of matrix metalloproteinases in renal pathophysiologies. American Journal of Physiology.-Renal Physiology. 2007;292:F905–F911.

Field ML, Khan O, Abbaraju J, Clark JF. Functional compartmentation of glycogen phosphorylase with creatine kinase and Ca2+ ATPase in skeletal muscle. J Theor Biol. 2006;238(2):257–68.

Epub 2005/07/12.

Al-Hail N, Butler AE, Dargham SR, Abou SA, Atkin SL. Creatine kinase is a marker of metabolic syndrome in qatari women with and without polycystic ovarian syndrome. Front Endocrinology (Lausanne). 2019;10,659. Epub 2019/10/15.

Frank M, Finsterer J. Creatine kinase elevation, lactacidemia, and metabolic myopathy in adult patients with diabetes mellitus. Endocr Pract. 2012;18(3):387–393.

Leask A, Abraham DJ. All in the CCN family: Essential matricellular signalling modulators emerge from the bunker. Journal of Cell Science. 2006;119:4803-4810. Available:

Kotb AS, Abdel-Hakim S, Ragy M, Elbassuoni E, Abdel-Hakeem E. Metformin ameliorates diabetic cardiomyopathy in adult male albino rats with type 2 diabetes. Minia Journal of Medical Research. 2022;33(4):128-138.

Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J. 2016;24(5):547 553. DOI: 10.1016/j.jsps.2015.03.013