Impact of Palm Oil Tainted Feed on Liver and Kidney Health in Wistar Rats: A Sub Chronic Study

Kanayo Mercy Odia *

Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port-Harcourt, PMB 5323, Nigeria.

M. Aniyeloye

Animal Research Unit, Pharmacology Department, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port-Harcourt, PMB 5323, Nigeria.

*Author to whom correspondence should be addressed.


Background: Palm oil is an edible vegetable oil obtained from the mesocarp (reddish pulp) of the fruit of the oil palm trees. The present study evaluated the effects of sub-chronic consumption of palm oil-tainted feed (POTF) on hepatorenal parameters in male Wistar rats.

Methods: Twenty-five (25) male Wistar rats with an average weight of 150±15g were used for the study. They were randomly selected into 5 groups of 5 rats each. Group 1 controlled and accessed only normal feed and water ad libitum. Groups 2 and 3 were fed with 10% and 20% POTF and water ad libitum respectively. Groups 4 and 5 were fed with 10% and 20% POTF + 0.02mg/ml and 0.04mg/ml of simvastatin respectively, and water ad libitum respectively. After 60 days of exposure/consumption of the POTF; blood samples were obtained from the study animals via cardiac puncture after proper sedation. The obtained data were analyzed with IBM SPSS version 25.

Results: The serum level of a liver enzyme-alanine transaminase (ALT) had no significant (P>0.05) change in all the test groups (groups 2, 3, 4, and 5) when compared to the control group. The level of aspartate transaminase (AST) was found to be significantly (p<0.05) raised in test group 3 when compared to that of group 1 (control). The alkaline phosphatase (ALP) level was significantly (p<0.05) reduced in test group 3 but markedly (p>0.05) elevated in test group 4 when compared to group 2. The serum levels of total protein, albumin, and total bilirubin indicated no significant (P>0.05) difference except for conjugated bilirubin which was significantly (p<0.05) reduced in test group 2. 

Conclusion: Except for greater doses, which can have some potentially harmful consequences, mild sub-chronic POTF consumption (10%) in the study animals did not negatively impact any liver enzymes or certain renal indices in the study models.

Keywords: Sub-chronic consumption, palm oil tainted feed, hepatorenal parameters, moderate use of safe, annatural palm oil

How to Cite

Odia, Kanayo Mercy, and M. Aniyeloye. 2024. “Impact of Palm Oil Tainted Feed on Liver and Kidney Health in Wistar Rats: A Sub Chronic Study”. International Research Journal of Gastroenterology and Hepatology 7 (1):44-50.


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Popkin BM. Global nutrition dynamics: The world is shifting rapidly toward a diet linked with noncommunicable diseases. The American Journal of Clinical Nutrition. 2006;84(2):289-98.

Jia Q, Xia Y, Zhang Q, Wu H, Du H, Liu L, Wang C, Shi H, Guo X, Liu X, Li C. Dietary patterns are associated with prevalence of fatty liver disease in adults. European Journal of Clinical Nutrition. 2015;69(8):914-21.

Li T, Guan L, Wang X, Li X, Zhou C, Wang X, Liang W, Xiao R, Xi Y. Relationship between dietary patterns and chronic diseases in rural population: management plays an important role in the link. Frontiers in Nutrition. 2022;9:866400.

Panagiotakos DB, Pitsavos C, Skoumas Y, Stefanadis C. The association between food patterns and the metabolic syndrome using principal components analysis: The ATTICA Study. Journal of the American Dietetic Association. 2007;107(6):979-87.

Achaw OW, Danso-Boateng E. Manufacture of crude palm oil and refined palm oil. InChemical and Process Industries: With examples of industries in Ghana. Cham: Springer International Publishing. 2021;10:195-211.

Raji RO, Inengite AK, Godwin J, Ajibesin KK. Assessment of proximate and physicochemical properties of crude palm oil from south-west and South-south Nigeria. African Journal of Pure and Applied Chemistry. 2022;16(2):40-56.

Edo GI, Makinde MG, Nwosu LC, Ozgor E, Akhayere E. Physicochemical and pharmacological properties of palm oil: an approach for quality, safety, and nutrition evaluation of palm oil. Food Analytical Methods. 2022;15(8):2290-305.

Imoisi OB, Ilori GE, Agho I, Ekhator JO. Palm oil, its nutritional and health implications. Journal of Applied Sciences and Environmental Management. 2015;19(1):127-33.

Mancini A, Imperlini E, Nigro E, Montagnese C, Daniele A, Orrù S, Buono P. Biological and nutritional properties of palm oil and palmitic acid: Effects on health. Molecules. 2015;20(9):17339-61.

Siri-Tarino PW, Sun Q, Hu FB, Krauss RM. Saturated fatty acids and risk of coronary heart disease: modulation by replacement nutrients. Current atherosclerosis reports. 2010 Nov;12:384-90.

DiNicolantonio JJ, Lucan SC, O’Keefe JH. The evidence for saturated fat and sugar related to coronary heart disease. Progress in cardiovascular diseases. 2016;58(5):464-72.

Reiner Ž. Hypertriglyceridaemia and risk of coronary artery disease. Nature Reviews Cardiology. 2017;14(7):401-11.

Mortensen MB, Caínzos-Achirica M, Steffensen FH, Bøtker HE, Jensen JM, Sand NP, Maeng M, Bruun JM, Blaha MJ, Sørensen HT, Pareek M. Association of coronary plaque with low-density lipoprotein cholesterol levels and rates of cardiovascular disease events among symptomatic adults. JAMA network open. 2022;5(2):e2148139.

Hsueh CJ, Wang JH, Dai L, Liu CC. Determination of alanine aminotransferase with an electrochemical nano Ir-C biosensor for the screening of liver diseases. Biosensors. 2011;1(3):107-17.

Zhang P, Wang CY, Li YX, Pan Y, Niu JQ, He SM. Determination of the upper cut-off values of serum alanine aminotransferase and aspartate aminotransferase in Chinese. World Journal of Gastroenterology: WJG. 2015;21(8): 2419.

Ezejindu DN, Chinweife KC, Ihentuge CJ. The effects of moringa extract on liver enzymes of carbon tetrachloride-induced hepatotoxicity in adult wister rats. Int J Eng Sci. 2013;2(7):54-9.

National Research Council. Guidelines of the National Institute of Health (NIH) for care and use of laboratory/research animals. [Retrieved in November; 2019].adults. JAMA network open. 2022 Feb 1;5(2):e2148139.

Odia OJ, Ofori S, Maduka O. Palm oil and the heart: a review. World journal of cardiology. 2015 Mar 3;7(3):144.

Ibrahim, N.I., Fairus, S. and Naina Mohamed, I. The effects and potential mechanism of oil palm phenolics in cardiovascular health: a review on current evidence. Nutrients. 2020;12(7):2055.

Sun Y, Neelakantan N, Wu Y, Lote-Oke R, Pan A, van Dam RM. Palm oil consumption increases LDL cholesterol compared with vegetable oils low in saturated fat in a meta-analysis of clinical trials. The Journal of nutrition. 2015 Jul 1;145(7):1549-58.

Abdulwaliyu I, Okoduwa SI, Sangodare R, Arekemase SO, Muhammad A. Review of studies on palm-oil consumption in relation to risk of cardiovascular diseases. Journal of Nutrition and Food Security. 2022.

Ozer J, Ratner M, Shaw M, Bailey W, Schomaker S. The current state of serum biomarkers of hepatotoxicity. Toxicology. 2008;245(3):194-205.

Gowda S, Desai PB, Hull VV, Avinash AK, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. The Pan African Medical Journal. 2009;3.

Kalas MA, Chavez L, Leon M, Taweesedt PT, Surani S. Abnormal liver enzymes: A review for clinicians. World journal of hepatology. 2021 Nov 11;13(11):1688.

Sharma U, Pal D, Prasad R. Alkaline phosphatase: an overview. Indian J Clin Biochem. 2014;29(3):269-78. DOI: 10.1007/s12291-013-0408-y. Epub 2013 Nov 26. PMID: 24966474; PMCID: PMC4062654.

Verma P. Alkaline phosphatase: A review article. International Journal of Biochemistry and Biomolecules. 2022;8(1): 23-8.

Riancho JA. Diagnostic approach to patients with low serum alkaline phosphatase. Calcified Tissue International. 2023;112(3):289-96.

Miller LL, Bly CG, Watson ML, Bale WF. The dominant role of the liver in plasma protein synthesis: A direct study of the isolated perfused rat liver with the aid of lysine-ε-C14. The Journal of experimental medicine. 1951;94(5):431.

Reeve EB, Pearson JR, Martz DC. Plasma protein synthesis in the liver: method for measurement of albumin formation in vivo. Science. 1963;139(3558):914-6.

De Feo P, Lucidi P. Liver protein synthesis in physiology and in disease states. Current Opinion in Clinical Nutrition & Metabolic Care. 2002;5(1):47-50.

Wang X, Chowdhury JR, Chowdhury NR. Bilirubin metabolism: applied physiology. Current Paediatrics. 2006;16(1):70-4.

Sticova E, Jirsa M. New insights in bilirubin metabolism and their clinical implications. World Journal of Gastroenterology: WJG. 2013;19(38):6398.

Naito M, Hasegawa G, Ebe Y, Yamamoto T. Differentiation and function of Kupffer cells. Medical Electron Microscopy. 2004;37:16-28.