Effect of High Salt Diet and Habiscus sabdariffa (Zobo) on the Histology of the Aorta in Wistar Rats
Published: 2023-09-04
Page: 108-120
Issue: 2023 - Volume 6 [Issue 1]
Darlington Akukwu
David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria.
Ugochukwu Aguwa *
Department of Anatomy, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
Catherine Akukwu
David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria.
Ambrose Agulanna
Department of Anatomy, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
Obatavwe Ukoba
Delta State University Teaching Hospital, Abraka, Nigeria.
Izuchukwu Okonkwo
Nnamdi Azikiwe University Teaching Hospital Nnewi, Nigeria.
Adline Aguwa
Nnamdi Azikiwe University Teaching Hospital Nnewi, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Effect of salt diet and hibiscus sabdoriffa (zobo) on the histology of the aorta of Wistar rats was investigated. A total of 20 adult Wistar rats weighing 162-220 g were randomly assigned into four groups (l-IV) of five animals each. Group I served as the control , group II received 8g of salt, group III received 8gm of salt with 600ml of zobo while group IV received 600ml of zobo . The salt was administered in 100g of feed for eight weeks, at the end of which blood was obtained for haematological analysis and sacrificed to obtain the aorta for histological analysis. The results obtained in this study showed a significant decrease in the body weight of rats taking high salt and zobo and an increase in body weight in rats taking normal diet and zobo compared to the control. The histological architecture of the aorta in the group taking salt and water shows a large thick wall and a moderate thickening in group taking salt and zobo. There is an increase level of AST in rats that received salt compared with Group I, III, IV and shows no significant changes in PCV and HB between the groups (l-IV). This study showed that high salt diet increased the blood pressure and volume in form of increase amount of water intake. It also caused thickening of the wall of the aorta.
Keywords: Salt, diet, zobo, Hibiscus sabdariffa
How to Cite
References
Khosh F, Khosh K. A natural approach to hypertension. Alternating Med. 2001;6:59-600.
Obatomi DK, Bikomo EO, Temple UJ. Antidiabetic properties of African mistletoe in streptozolocin-induced diabetic rats. J Ethnopharmacol. 1994;43:13-7.
Gu JW, Anand V, Shek EW, Moore MC, Brady AL. elly WC, Adair TH. Hypertension. sodium induces hypertrophy of cultural myocardial myoblasts and vascular smooth muscle cells. 1998;31:1083-7.
Sacks FM, Svetkey LP, Vollmer WM, Appel LJ, Brady GA, Harsha D, et al. SASH-sodium Collaborative Research Group. Effects on blood pressure of reduced dietary sodium and the dietary approaches to stop hypertension (DASH) diet N. English. J Med. 2001;344:3-10.
Aviv A. Salt and hypertension. The debate that begs the bigger question; Arch International Medicine. Arch Intern Med. 2001;161(4):507-10.
Frolich ED, Varagic J. Sodium directly impairs target organ function in hypertension^Curr Opinion Cardiology. 2005;20:424-9.
Meneton P, Jeunemaitre X, de Wardener HE, MacGregor GA. Links between dietary salt intake, renal salt handling, Blood pressure, and cardiovascular diseases. Physiol Rev. 2005: Dietary salt in take, renal salt handling;85(2):679-715.
Sandler AB, Johnson DH, Herbst RS. Anti-vascular endothelial growth factor monoclonal in non-small cell lung cancer. Clin Cancer Res. 2004;10(12 Pt 2):4258s-62s.
Zangari M, Anaissie E, Stopeck A, Morimoto A, Tan N, Cancet J, et al. a small molecular vascular endothelial growth factor tyrosine kinases receptor indubitor, in patients with refractory multiple myeloma. Clin Rez; phase II study of SU 2004;5416;10:88-95.
Sanders PW, Gibbs CL, Akhi KM, MacMillan-Crow LA, Zinn KR, Chen YF, et al. Increased dietary salt accelerates chronic allograft nephropathy in rats. Kidney Int. 2001;59(3):1149-57.
Ying W-Z, Sanders PW. Dietary salt modulates renal production of transforming growth factor-B n rat aortic endothelium. Am J Plugs. 1998;277(4 pt 2):H1293-8.
Chen PY, Sanders PW. L-arginine abrogates salt-sensitive hypertension in Dahl rats. J Clin Invest. 1991;88(5): 1559-67.
Redgrave J, Rabinowe S, Hollenberg NK, Williams GH. Correction of abnormal renal blood flow response to angiotensin II by converting enzyme inhibition in essential hypertensives. J Clin Invest. 1985; 75(4):1285-90.
Adegunloye BJ, Owolabi OA, Ajagbonna OP, Sofola OA, Coker HA. Mechanism of the blood lowering effect of the calyx extract of Hibiscus sabdariffa in rats. Afr J Med Sci. 1996;25:235-8.
Obiefuna PCM, Owolabi OA, Adegunloye BJ, Obiefuna IP, Sofola OA. The petal extract of hibiscus sabdariffaproduces relaxation of isolated rat Gorta. Int J Pharmacol. 1994;34:67-74.
Dikko M. Antihypertensive effects of Hibiscus sabdariffa calyx extract. In salt induced hypertension due to chronic nitric oxide synthetase (NOS) inhibition [M.Sc. thesis]. Sokoto, Nigeria: Usman Danfodiyo University; 2003.
Lawai SO, Falase AO. The effect of hypertension on the heart of adult Nigerians. Trop Cardiol. 1988;14:153-8.
Balogun MO, Ladipo OA. Circulatory responses to dynamic exercise in healthy adult Nigerians with or without parental hypertension. Trop Cardiol. 1998;14: 165-8.
Sofowara EA. Medicinal plants and traditional medicine in Africa. New York: John Wiley & Sons. 1982;22.
Ruiz-Ortega M, Rodríguez-Vita J, Sanchez-Lopez E, Carvajal G, Egido J. TGF- beta signaling in vascular fibrosis. Cardiovasc Res. 2007;74(2):196-206.
Saladin SK, Leslie M. Circulating system: blood vessels and circulation. Anatomy and physiology. 3rd ed. 2004;20: 749-50.
Sanders PW. Dietary salt intake, salt sensitivity, and cardiovascular health. Hypertension. 2009;53(3):442-5.
Sanders PW. Salt intake, endothelial cell signalling and progression of kidney disease. Hypertension. 2004;43(2):142-6.
Seymour EM, Singer AA, Bennink MR, Parikh RV, Kirakosyan A, Kaufman PB, et al. Chronic intake of a phytochemical-enriched diet reduces cardiac Fibrosis and diastolic dysfunction caused by prolonged salt-sensitive hypertension. J Gerontol A Biol Sci Med Sci. 2008;63(10):1034-42.
Stamler J, Rose G, Elliot P, Dyer A, Marmount M, Kestelot H, Starmler R. Findings of the international cooperative intersalt study. Hypertension. 17, Suppl-1:9-15.
Staessen JA, Kuznetsova T, Zhang H, Maillard M, Bochud M, Hasenkamp S, et al. Blood pressure and renal sodium handling in relation to genetic variation in the DRD1 promoter and GRK4. Hypertension. 2008;51(6):1643-50.
Tolins JP, Shultz PJ. Endogenous nitric oxide synthesis determines sensitivity to the pressure effect of salt. Kidney Int. 1994;46(1):230-6.
Intersalt. An international study of electrolyte excretion and blood pressure. Results of 24h urinary sodium and potassium excretion. Br Med J. 1988;297:319-28.
Dahl LK, Heine M, Tassinari L. Effects of chronic excess salt ingestion. J Exp Med. 1962;115(6):1173-90.
Ferrari AU, Mark AL. Sensitization of aortic bar receptors by high salt diet in Dahl salt-resistant rats. Hypertension. 1987;10:5560.
Mulvany MJ, Hansen DK, Aalkjaer C. Direct evidence that the greater contractility of resistance vessels in SH rats is associated with narrowed limen and thickened media and an increased no. of smooth muscle cell layer. Circ Res. 1978;43:854-64.
Adegunloye BJ, Sofola OA. Effects of dietary salt loading and high calcium diet on vascular smooth muscle responses and endothelial function in rats. Clin Exp Pharmacol Physiol. 1997;24(11):814-8.
Nishida Y, Ding J, ZHour M, Chen O, Murakami H, Wu XZ, et al. Role of nitric oxide in vascular hyper-responsiveness to norepinephrine in hypertensive Delil rats. J Hypertens. 1998;16:1611-8.
Obiefuna PCM, Sofola OA, Ebeigbe AB. Dietary salt loading attenvate endothelium-dependent relaxation in response to histamine but hot to acetylcholine in rats aortic rings. Exp Physiol. 1991;76(1): 135-8.
Lenda DM, Sauls BA, Boegehold MA. Reactive oxygen species may contribute to reduced endothelium-dependent dilation in rats fed high salt. Am J Physiol Heart Circ Physiol. 2000;279(1):H7-H14.
Ebeigbe AB, Cressier F, Konneh MK, Luu TD, Criscione L. Influence of NA-momomethyl L. Biochem Biophys Res Commun. 1990;169(3):873-9.
Adeagbo AS, Triggle CR. Varying extra cellular (K+): a functioned approach to separating EDHF and EDNO-related mechanisms in perfused rat mesenteric arterial bed. J Cardiovasc Pharmacol. 1993;21(3):423-9.
Morris RCT, Sebastian A, forman A, Tanaka M, Schmidlin O. Normotensive salt sensitivity: effects of race and dietary potassium. Hypertension. 1999;33(1): 18-23.
Ojeda D. JimAOnez-Ferrer E, Zamilpa A, Herrera-Arellano A, Tortoriello J, Alvarez L. J Ethnopharmacol. Inhibition of Angiotensin converting enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa; 2009.
Sellin L, Stegbaver J, Dekomien G, Rump LC. Quack, vonend O. Atale of two patients with Mendelian hypertension. 2008;51:609-14.
Rodriguez-Iturbe B, Vazirl ND, Herrara-Acosta J, Johnson RJ. Oxidative stress, renal infiltration of immune cells, and salt-sensitive hypertension: all for one and one for all. Am J Physiol Ren Physiol. 2004;285:F606-16.
Vaziri ND, Herrera-Acosta J, Johnson RJ. Rodriguez-lturbe B. Am J Physiol Ren Physiol. Oxidative stress, renal infiltration of immune cells and salt-sensitive hypertension all for one and one for all. 2004;286-F:606-F. 616.
Sofola OA, Knill A, Hainsworth R, Drinkhill M. Change in endothelial function in mesenteric arteries of Sprague-Dawley rats fed a high salt diet. J Physiol. 2002;543(1):255-60.
Abudu J. 0 and Sofola DA. Niger J Physiol Sci. Relationship between red cell mass and packed cell volume in Nigerian Primigravidae. 1994;10(l&2):13-21.
Almeida FA, Suzuki M, Maack T. High salt intake increases the vasorelaxant effect of ANF on the isolated perfused rat kidney. Braz J Med Biol Res. 1991;24(11):1149-57.
Ardiansyah OY, Shirakawa H, Koseki T, Komai M. Novel effects of a single administration of ferulic acid on the regulation of blood; 2008.
pressure and the hepatic lipid metabolic profile in stroke-prone spontaneously hypertensive rats. J. Agricfood Chem. 56:2825-2830.
Burnier M. Ethnic differences in renal handling of water and solids in hypertension. Hypertension. 2008; 52(2):203-4.
Vanhoutte PM, Boulanger CM, Mombouli JV. Endothelium derived relaxing factors and converting enzymes inhibition. Am J Cardiol. 1995;76(15):3E-12E.
Chen CC, Hsu JD, Wang SF, Chiang HC, Yang MY, Kao ES, et al. Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits. J Agric Food Chem Aug 27. 2003; 51(18):5472-7.
Chun TY, Bankir L, Eckert GJ, Bichet DG, Saha C, Zaidi SA, et al. Ethnic differences in renal responses to furosemide. Hypertension. 2008;52(2):241-8.
Correa FMA, Viswanathan M, Ciuffo GM. Keisuke Tsutsume Juan M. Saavedra. Kidney Angiotensin II receptors and concerting enzyme in neonatal and adult wistar-Kyoto and spontaneously hypertensive rats. Peptide. 1995;16(1):19-24.
Gu JW, Tian N, Shparago M Tan W, Bailey AP, Manning RD. Jr Renal NF- KB activation and TNF & Up regulation correlate with salt-sensitive hypertension in Dahl Salt-sensitive rats. American Journal of physiololgy Regulation. Integrecomp physiology: 291.R. 2006;1817-RI1824.
Gu JW, Tian N, Shparago M, Tan W, Bailey AP, Manning Rd, Jr. Renal NF-kappa B activation and TNF-alpha up regulation correlate with saltsensitive hypertension in Dahl salt-sensitive rats. Am J Physiol Regul Integr Comp Physiol. 2006;291:R1317-824.
Gu D, Rice T, Wang S, Yang W, GU C, Chen CS, et al. [DP] Heritability of blood pressure responses to dietary sodium and potassium intake in a Chinese population. Hypertension. 2007;50(1):116-22.
Hall JE, Guyton AC, Smith MJ, Jr, Coleman TG. Renal luid electrolyte physiology. Pressure and renal function during chronic changes in sodium intake: role of Angiotensin. American Journal of physiology. 1980;239:F271-80.
Herrera-Arellano A, Miranda-Sánchez J, Avila-Castro P, Herrera-Alvarez S, Jiménez-Ferrer JE, Zamilpa A et al. Clinical effects produced by a standardized herbal medicinal product of Hibiscus sabdariffa on patients with hypertension. A randomized, double-blind, lisinopril-controlled clinical trial. Planta Med. 2007; 73(1):6-12. D
Johnson RJ, Herrara-Acosto J, Schreiner GF. Rodriguez-lturbe B. Subtle Acquire Ren Inj Mech Salt-Sens Hypertens N. Engl. J. med. 2002;346:913-23.
Juric D, Wojciechowski P, Das DK, Netticadan T. Prevention of concentric hypertrophy and diastolic impairment in aortic-banded rats treated with resveratol. Am J Physiol Heart Circ Physiol. 2007;292. H2143:H-2138.
Grove KL, Deschepper CF. High intake differentially regulates kidney angiotension IV AT4 receptors in Wister-Kyoto and Spontaneously hypertensive rats. Life Sci. 1999;64(20):1811-8.
Laurent S, Boutouyrie P. Recent advances in arterial stiffness and wave reflection in human hypertension. Hypertension. 2007; 49(6):1202-6.
Lifton RP, Gharavi AG, Geller DS. Molecular mechanisms of human hypertension. Cell. 2001;104(4):545-56.
Liu Z, Song Y, Zhang X, Liu Z, Zhang W, Mao W et al. Effects of trans-resveratrol on hypertension- induced cardiac hypertrophy using the partially rephrectomized rat model. Clin Exp Pharmacol Physiol. 2005;32(12):1049-54.
Luft FC, Miller J. Grm CE, fineberg NS, Christian JC, Daugherty SA, Weinberges MA. Hypertension; 17. Salt sensitivity and resistance of blood pressure. Age and race as factors in physiological responses. 1997;1108:IL02.
Moore KL, persaud TV. The developing Human: clinically oriented Embryology. 6th ed. Philadelphia: saunders; 1998.
Subramaniam V, Lip GY. Hypertension to heart failure: A pathophysiological spectrum relating blood pressure, drug treatments and stroke. Expert Rev Cardiovasc Ther. 2009;7(6):703-13.
Arguedas JA, Perez MI, Wright JM. Treatment blood pressure targets for hypertension. Cochrane Database Syst Rev. 2009;3(3):CD004349.
Masson GMC, Aoki K. Page IH. Effects of sinoaortic denervation on renal and adrenal hypertension. Am J Physiol. 1966;211:94-104.
Dzielak DJ, Norman RA, Jr. Renal nerves are not necessary for onset or maintenance of DOC-salt hypertension in rats. Am J Physiol. 1985;249(5 Pt 2): H945-9.
Elliott P, Walker LL, Little MP, Blair-West JR, Shade RE, Lee DR et al. Change in salt intake affects blood pressure of chimpanzees: implications for human populations. Circulation. 2007;116(14): 1563-8.
Dacie JF, Lewis SM. Practical haematology. 6th ed. Edinburgh, Melbourne and New York: Churchill livingstone. 1984;24-36.
Spech HJ, Lieh H. Gastroenterology volume. 1983;(21).
Redgreeve J, Rabinowe S, Hollemberg NK, Williams GH. Correction of abnormal renal blood flow responses to angiotensin II by converting enzymes in essential hypertension. J Clin Invest. 1985;1285-1290:75.
Greene AS, Yu ZY, Roman RJ, Cowley AW. Role of blood volume expansion in Dahl rat model of hypertension. Am J Physiol; (2pt2). 1990;258(2 Pt 2): H508-14.
Bech JN, Nielsen CB, Ivarsen P, Jensen KT, Pedersen EB. Dietary sodium affects systemic and renal hemodynamic responses to NO inhibition in healthy humans. Am J Physiol. 1998;274(5 pt 2):F914-23.
Cowley AW, Skelton MM, Merrill DC, Quillen EW, Switzer SJ. Influence of daily sodium intake on vasopressin secretion and drinking in dogs. Am J Physiol. 1983;245(6):R860-72.
Luft FC, Fineberg NS, Sloan RS, Hunt JN. The effect of dietary sodium and protein on urine volume and water intake. J Lab Clin Med. 1983;101(4):605-10.
Hladky SB, Rink TJ. Osmoregulation, In Hladky SB, rink teds; body fluid and kidneys physiology. Baltimore. Med. 1986:143-77.
He FJ, Markandu ND, Sagnella GA, MacGregor GA. Effect of salt intake on renal excretion of water in humans. Hypertension. 2001;38(3):317-20.
Guyton AC. Blood pressure control- special role of the kidneys and body fluids. Science. 1991;252(5014):1813-6.
Weinberger MH. Sensitivity of blood pressure in human. Hypertension. 1996;27(3 Pt 2):481-90.
Lifton RP. Molecular genetics of human blood pressure variations. Science. 1996;272(5262):676-80.
Melissa P. Liver enzymes, Dr: ”Melissa Palmer’s Guide of hepatitis and liver disease”. (516). 2004;2626:939.
Turner ST, Bailey KR, Fridley BL, Chapman AB, Schwartz GL, Chai HS, et al. Genoric association analysis suggests chromosome 12 locus influencing antihypertensive response to thiazide diuretic. Hypertension. 2008;52(2): 359-65.
Vaziri ND. Rodriguez-lturbe B. 2006: Mechanisms of disease. Oxidative stress and inflammation in the pathogenesis of hypertension: Nature Clinical Practical Nephrology;2:582-93.
Wahabi HA, Alansary LA, Al-Sabban AH, Glasziuo P. The effectiveness of Hibiscus sabdariffa in the treatment of hypertension: A systematic review. Phyto-medicine: Oct 2. Pub Med – as supplied by publisher; 2009.
Weinberger MH, Fineberg NS, Fineberg SE, Weinberger M. Salt sensitivity, pulse pressure, and death in normal and hypertensive humans. Hypertension. 2001;37(2 Pt 2):429-32.
Ying WZ, Aaron K, Sanders PW. Mechanism of dietary salt-mediated increase in intravascular production of TGF-B1. Am J Physiol Renal Physiol. 2008;295(2):F406-14.
Ying WZ, Sanders PW. Dietary salt increases endothelial nitric oxide syntheses and TGF-B1 in rats’ aortic endothelium. Am J Physiol. 1999;277(4 pt 2):H1293-8.
Ying WZ, Sanders PW. Dietary salt modulates renal production of transforming growth factor-B in rats. Am J Physiol. 1999;274(4 pt 2):F635-41.
Yu HCM, Burrell LM, Black MJ, WULL DRJ, Copper ME, Johnston CJ; 1998.
Salt induces myocardial and renal fibrosis in normotensive and hypertensive rats. Circulation; 98: 2621-2628.
Yu HC, Burrell LM, Black MJ, Wu LL, Dilley RJ, Cooper ME, et al. Salt induces myocardial and renal fibrosis in normotensive and hypertensive rats. Circulation. 1998;98(23):2621-8.
Capelli H, Ross D, Somerville J. Aortic regurgitation in tetrad of Fallot and pulmonary atresia. Am J Cardiol. 1982;49(8):1979-83.
Marelli AJ, Perloff JK, Child JS, Laks H. Pulmonary atresia with ventricular septal defect in adults. Circulation. 1994; 89(1):243-51.