Phytochemical Screening and Evaluation of the Anti Ulcer Activity of Dacryodes edulis (Burseraceae) Leaf Extracts in Wistar Rats
Published: 2023-04-10
Page: 53-66
Issue: 2023 - Volume 6 [Issue 1]
Nomo Honore
Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Cameroon.
Tembe Estella Fokunang *
Department of Pharmacotoxicology and Pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Cameroon.
Bayaga Herve
Department of Pharmacotoxicology and Pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Cameroon.
Njinkio Nono Borgia
Department of Pharmacotoxicology and Pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Cameroon.
Mbacham Wilfred
Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Cameroon.
Charles Fokunang
Department of Pharmacotoxicology and Pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Cameroon.
*Author to whom correspondence should be addressed.
Abstract
Peptic ulcer disease is a chronic condition of the gastrointestinal tract that results from an imbalance between protective and aggressive factors of the gastric mucosa. Many synthetic drugs are used for the treatment of this disease, but they are costly and exhibit limited efficacy, and could cause adverse effects. Hence, the present study aimed to screen the phytochemicals and evaluate the anti-ulcer activities of Dacryodes edulis (G. Don) H.J. Lam leaf extracts in Wistar rats. Four leaf extracts obtained by aqueous cold maceration, hydroethanol maceration, infusion and decoction respectively were used. Qualitative and quantitative phytochemical screening was carried out and an ulceration model based on HCl/ethanol mixture (150mM/60%) was used to evaluate the gastroprotective activity in vivo at a dose of 500mg/kg. The physiological and biochemical parameters of the homogenate were evaluated. Qualitative analysis showed the presence of total polyphenols, tannins, flavonoids, flavonols, saponosides, coumarins, anthraquinones, cardiac glycosides and ascorbic acid. Quantitative analysis showed a predominance of flavonoids followed by flavonols, polyphenols and tannins. The extracts obtained by hydro-ethanolic, and aqueous maceration showed the best gastroprotective activity with percentages of 81.61% and 75% respectively. The aqueous extracts obtained by infusion and decoction showed a low activity (52% and 26% respectively). The biochemical analysis of the gastric juice content showed a significant increase in the activity of antioxidant enzymes (Catalase, SOD, GSH) and a pronounced decrease in MDA in all groups. We concluded that D. edulis leaf extracts showed potential bioactive metabolites predorminantly of flavonoids, flavonols, polyphenols and tanins, numerous natural antioxidants that was responsible for the observed gastroprotective activity and this plant could be a promising source of a more effective and safer antiulcer drug. This study also confirm the use of D. edulis as a category 1 improved traditional medicine, while in development for a category 2 phytomedicine.
Keywords: Dacryodes edulis, antiulcer, phytochemicals, antioxidant, wistar rat
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Gogola D. Phytochemical screening, antioxidant and gastro-protective activity studies on the fruit peels of selected varieties of Banana. Herbal Medicines Journal. Available:http://hmj.lums.ac.ir/index.php/hmj/article/view/763
Farrokhi Yekta R, Amiri-Dashatan N, Koushki M, Dadpay M, Goshadrou F. A metabolomic study to identify potential tissue biomarkers for indomethacin-induced gastric ulcer in rats. Avicenna J Med Biotechnol [Internet]. 2019;11(4): 299‑307. Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925398/Access on 6 déc 2020
Gogola D. Phytochemical screening, antioxidant and gastro-protective activity studies on the fruit peels of selected varieties of Banana. Herbal Medicines Journal [Internet]. Available:http://hmj.lums.ac.ir/index.php/hmj/article/view/763
Kuna L, Jakab J, Smolic R, Raguz-Lucic N, Vcev A, Smolic M. Peptic ulcer disease: A brief review of conventional therapy and herbal treatment options. Journal of Clinical Medicine. Available:https://www.mdpi.com/2077-0383/8/2/179
Gilard M, Arnaud B, Le Gal G, Abgrall JF, Boschat J. Influence of omeprazol on the antiplatelet action of clopidogrel associated to aspirin. J Thromb Haemost. 2006;4(11): 2508‑9.
Palle S, Kanakalatha A, Kavitha CHN. Gastroprotective and antiulcer effects of Celastrus paniculatus seed oil against several gastric ulcer models in rats. Journal of Dietary Supplements [Internet]. 2018;15(4):373‑85.
Available:https://www.tandfonline.com/doi/full/10.1080/19390211.2017.1349231 Access on 15 oct 2022
Ghebremariam YT, Lee JC, LePendu P, Erlanson DA, Slaviero A, Shah NH, et al. Response to letters regarding article, « unexpected effect of proton pump inhibitors: elevation of the cardiovascular risk factor asymmetric dimethylarginine ». Circulation. 2014;129(13):e428.
Falcão HS, Mariath IR, Diniz MFFM, Batista LM, Barbosa-Filho JM. Plants of the American continent with antiulcer activity. Phytomedicine. 2008;15(1‑2): 132‑46.
Dacryodes edulis (G.Don) H. J. Lam.
Available:https://www.prota4u.org/database/protav8.asp?fr=1&g=pe&p=Dacryodes+edulis+(G.Don)+H.J.Lam
Olivier TT, Moïse F, Jackson SA, Francis NT. A review on traditional uses, phytochemical and pharmacological profiles, spiritual and economic values, and toxicity of Dacryodes edulis (G. DON) H. J. LAM. Journal of Drug Delivery and Therapeutics [Internet]. Available:http://www.jddtonline.info/index.php/jddt/article/view/1276
Sama Fonkeng L, Mouokeu RS, Tume C, Njateng GSS, Kamcthueng MO, Ndonkou NJ, et al. Anti-Staphylococcus aureus activity of methanol extracts of 12 plants used in Cameroonian folk medicine. BMC Res Notes. 2015;8:710.
Ufelle S, Ukaejiofo E, Achukwu P, Eluke B, Ghasi S, Neboh E. Potential haemopoietic effects of Dacryodes edulis seeds extract in wistar rats. 2015;3:2348‑5728.
Sofowora A. Medicinal plants and traditional medicine in Africa New York. John Willey and sons. 1982;107‑12.
Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. Springer Science & Business Media. 1998:504.
Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med. Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524761/
Shier David, Lewis Ricki, Butler Jackie, Hole John. Hole’s human anatomy and physiology. Hole’s Human Anatomy & Physiology. 9th ed. McGraw-Hill, Boston; 2001.
Hole’s Human Anatomy & Physiology: 9th Edition by John W Jackie; Hole - Hardcover - 9th Edition - 2001 - from A2zbooks (SKU: ABE395302710). Available:https://www.biblio.com/book/holes-human-anatomy-physiology-9th-edition/d/313146750
Makkar H. Measurement of total phenolics and tannins using folin-ciocalteu method. 2003:49‑51.
Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med. Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3524761/
Miliauskas G, van Beek TA, Venskutonis PR, Linssen JPH, de Waard P, Sudhölter EJ. Antioxidant activity of Potentilla fruticosa. Journal of the Science of Food and Agriculture. Available: https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.1914
Ali-Rachedi F, Meraghni S, Touaibia N, Mesbah S. Analyse quantitative des composés phénoliques d’une endémique algérienne Scabiosa atropurpurea sub. Maritima L. Bull Soc R Sci Liege.
Available:https://popups.uliege.be/0037-9565/index.php?id=7398
Waterborg JH. The lowry method for protein quantitation. In: Walker JM, éditeur. The Protein Protocols Handbook [Internet]. Totowa, NJ: Humana Press (Springer Protocols Handbooks). 2009:7‑10. Available:https://doi.org/10.1007/978-1-59745-198-7_2 Access on 26 juin 2022
Zollinger-Ellison Syndrome: Background, Pathophysiology, Etiology; 2021;
Available:https://emedicine.medscape.com/article/183555-overview Access on 22 oct 2022
Fashner J, Gitu AC. Diagnosis and treatment of peptic ulcer disease and h. pylori infection. Am Fam Physician. 2015;91(4):236‑42.
Bi WP, Man HB, Man MQ. Efficacy and safety of herbal medicines in treating gastric ulcer: A review. World J Gastroenterol. 2014;20(45):17020‑8.
Prabhakar PV, Reddy UA, Singh SP, Balasubramanyam A, Rahman MF, Indu Kumari S, et al. Oxidative stress induced by aluminum oxide nanomaterials after acute oral treatment in wistar rats. J Appl Toxicol. 2012;32(6):436‑45.
Ellman GL. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics [Internet]. 1959;82(1):70‑7.
Available:https://www.sciencedirect.com/science/article/pii/0003986159900906
Access on 29 oct 2022
Gong G, Rigetu Zhao, Zhu Y, Yu J, Wei B, Xu Y, et al. Gastroprotective effect of cirsilineol against hydrochloric acid/ethanol-induced gastric ulcer in rats. Korean J Physiol Pharmacol [Internet]. 2021;25(5):403‑11.
Available:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405436/Access on 19 juin 2022
Zhou D, Yang Q, Tian T, Chang Y, Li Y, Duan LR, et al. Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role. Biomed Pharmacother. 2020;126:110075.
Aponjolosun BS, Fasola TR. Phytochemical, antimicrobial and toxicity assessment of Dacryodes edulis (G. Don.) H. J. Lam. Leaf Extracts. African Journal of Biomedical Research [Internet]. 2022; 25(1):101‑6.
Available:http://ojshostng.com/index.php/ajbr/article/view/227 Access on 19 juin 2022
Uzor PF, Onyishi CK, Omaliko AP, Nworgu SA, Ugwu OH, Nwodo NJ. Study of the antimalarial activity of the leaf extracts and fractions of Persea americana and Dacryodes edulis and their HPLC analysis. Evidence-Based Complementary and Alternative Medicine [Internet]. 2021;2021: e5218294. Available:https://www.hindawi.com/journals/ecam/2021/5218294/Access on 7 sept 2021
Antony A, Farid M. Effect of temperatures on polyphenols during extraction. Applied Sciences [Internet]. 2022;12(4):2107. Available:https://www.mdpi.com/2076-3417/12/4/2107 Access on 20 juin 2022
Enengedi I, Ekpa O, Akpabio U. Antioxidant and free radical scavenging properties of Dacryodes edulis leaf and bark extracts. International Journal of Herbal Medicine.
Nwaonukuru E, Olaniyi OO, Oluwole FS. Possible gastroprotective mechanisms of Dacryodes edulis extract in indomethacin-induced gastric ulceration in male wistar rats. Journal of Pharmaceutical Research International [Internet]. 2018;1‑12. Available:https://journaljpri.com/index.php/JPRI/article/view/19120
Access on 19 juin 2022
Biswas K, Bandyopadhyay U, Chattopadhyay I, Varadaraj A, Ali E, Banerjee RK. A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical *. Journal of Biological Chemistry [Internet]. 2003;278(13): 10993‑1001. Available:https://www.jbc.org/article/S0021-9258(19)32374-9/abstract Access on 28 oct 2022
Odo CE. Anti-ulcerogenic evaluation of the fractions of the ethanol layer of the chloroform-ethanol extract of the leaves of Dacryodes edulis. Afr J Pharm Pharmacol [Internet]. 2017;11(1):10‑6. Available:http://academicjournals.org/journal/AJPP/article-abstract/E9EFE7C62211 Access on 20 juin 2022
Sies H, Belousov VV, Chandel NS, Davies MJ, Jones DP, Mann GE, et al. Defining roles of specific Reactive Oxygen Species (ROS) in cell biology and physiology. Nat Rev Mol Cell Biol [Internet]. 2022;1‑17.
Available:https://www.nature.com/articles/s41580-022-00456-z Access on 19 juin 2022